The customized Square media bottle, as the name suggests, is used to culture cells. According to the material, it can be divided into glass and plastic, the bottom shape is square, rectangle, triangle, etc., according to the volume of the bottle, there are 5 to 500ml for selection. In addition, after the surface is specially modified, the cells can be better adherently cultured, so that they can be divided into 25-175cm2 according to the area of ​​cell adhesion.

 
Different shapes of customized Square media bottles have different uses. For culturing cells, a flat bottom is usually used, which is convenient for observation under the microscope, has a clear bottom area, and the height of the cell culture liquid is relatively consistent. Therefore, when doing experiments, whether it is adherent or suspended cells, a flat bottom plate is generally used. Be sure to use a flat-bottomed culture plate for absorbance measurements. Pay special attention to the material.
 
U-shaped or V-shaped, generally only used when some special requirements. For example, in immunology, when two different lymphocytes are mixed and cultured, they need to be stimulated by contact with each other. At this time, a U-shaped plate is generally used, because the cells will gather in a small range due to the effect of gravity. Round-bottom culture flasks are also used for isotope incorporation experiments that require a cell harvester to collect cell cultures, such as "mixed lymphocyte culture". Type V is commonly used for cell killing and immunological hemagglutination experiments. This experiment of cell killing can also be replaced by a U shape (after adding cells, low speed centrifugation).
 
The customized Square media bottle made in China generally has a closed lid without an inner pad. It is often used for airtight culture to ensure its airtightness; the imported culture bottles have many types of lids: airtight caps, polyester caps, breathable caps, Septum caps, etc. The performance of the airtight cap is the same as that of the domestic one. The polyester cap is often used for open culture, and the gas in the bottle can be exchanged with the gas in the environment by simply loosening the cap. The breathable cap is a 0.2μm hydrophobic membrane added to the cap to provide sterile gas exchange and reduce the risk of contamination. Commonly used for open culture, recommended for CO2 incubator culture, especially for experiments that require long-term culture. The septum cap has a pre-cut opening on the septum, which allows the nozzle to be inserted through the septum to add liquid, aspirate or harvest cells, reducing the chance of contamination and maintaining a closed and sterile environment for the customized Square media bottle.